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Efficient uniform isotope labeling of Abl kinase expressed in Baculovirus-infected insect cells.

Strauss, Andre and Bitsch, Francis and Fendrich, Gabriele and Graff, Patrick and Knecht, Rene and Meyhack, Bernd and Jahnke, Wolfgang (2005) Efficient uniform isotope labeling of Abl kinase expressed in Baculovirus-infected insect cells. Journal of Biomolecular NMR, 31 (4). pp. 343-349. ISSN 0925-2738

Abstract

This report shows for the first time the efficient uniform isotope labeling of a recombinant protein expressed using Baculovirus-infected insect cells. The recent availability of suitable media for (15)N- and (13)C/(15)N-labeling in insect cells, the high expression of Abl kinase in these labeling media and a suitable labeling protocol made it possible to obtain a (1)H-(15)N-HSQC spectrum for the catalytic domain of Abl kinase of good quality and with label incorporation rates > 90%. The presented isotope labeling method should be applicable also to further proteins where successful expression is restricted to the Baculovirus expression system.

Item Type: Article
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Additional Information: author can archive post-print (ie final draft post-refereeing); Publisher's version/PDF cannot be used
Keywords: Abl kinase; Baculovirus; HSQC; NMR; STI571; uniform isotope labeling
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Date Deposited: 14 Dec 2009 13:56
Last Modified: 31 Jan 2013 01:11
URI: https://oak.novartis.com/id/eprint/629

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