Efficient uniform isotope labeling of Abl kinase expressed in Baculovirus-infected insect cells.
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Strauss, Andre, Bitsch, Francis, Fendrich, Gabriele, Graff, Patrick, Knecht, Rene, Meyhack, Bernd and Jahnke, Wolfgang (2005) Efficient uniform isotope labeling of Abl kinase expressed in Baculovirus-infected insect cells. Journal of Biomolecular NMR, 31 (4). pp. 343-349. ISSN 0925-2738
Abstract
This report shows for the first time the efficient uniform isotope labeling of a recombinant protein expressed using Baculovirus-infected insect cells. The recent availability of suitable media for (15)N- and (13)C/(15)N-labeling in insect cells, the high expression of Abl kinase in these labeling media and a suitable labeling protocol made it possible to obtain a (1)H-(15)N-HSQC spectrum for the catalytic domain of Abl kinase of good quality and with label incorporation rates > 90%. The presented isotope labeling method should be applicable also to further proteins where successful expression is restricted to the Baculovirus expression system.
| Item Type: | Article |
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| Additional Information: | author can archive post-print (ie final draft post-refereeing); Publisher's version/PDF cannot be used |
| Keywords: | Abl kinase; Baculovirus; HSQC; NMR; STI571; uniform isotope labeling |
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| Date Deposited: | 14 Dec 2009 13:56 |
| Last Modified: | 31 Jan 2013 01:11 |
| URI: | https://oak.novartis.com/id/eprint/629 |