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SadA, a Trimeric Autotransporter from Salmonella enterica Serovar Typhimurium, Can Promote Biofilm Formation and Provides Limited Protection against Infection.

Raghunathan, Dhaarini and Wells, Timothy J and Morris, Faye C and Shaw, Robert K and Bobat, Saeeda and Peters, Sarah E and Paterson, Gavin K and Jensen, Karina Tveen and Leyton, Denisse L and Blair, Jessica M A and Browning, Douglas F and Pravin, John and Flores-Langarica, Adriana and Hitchcock, Jessica R and Moraes, Claudia T P and Piazza, Roxane M F and Maskell, Duncan J and Webber, Mark A and May, Robin C and Maclennan, Calman A and Piddock, Laura J and Cunningham, Adam F and Henderson, Ian R (2011) SadA, a Trimeric Autotransporter from Salmonella enterica Serovar Typhimurium, Can Promote Biofilm Formation and Provides Limited Protection against Infection. Infection and immunity, 79 (11). pp. 4342-4352. ISSN 0019-9567

Abstract

Salmonella enterica is a major cause of morbidity worldwide and mortality in children and immunocompromised individuals in sub-Saharan Africa. Outer membrane proteins of Salmonella are of significance because they are at the interface between the pathogen and the host, they can contribute to adherence, colonization, and virulence, and they are frequently targets of antibody-mediated immunity. In this study, the properties of SadA, a purported trimeric autotransporter adhesin of Salmonella enterica serovar Typhimurium, were examined. We demonstrated that SadA is exposed on the Salmonella cell surface in vitro and in vivo during infection of mice. Expression of SadA resulted in cell aggregation, biofilm formation, and increased adhesion to human intestinal Caco-2 epithelial cells. Immunization of mice with folded, full-length, purified SadA elicited an IgG response which provided limited protection against bacterial challenge. When anti-SadA IgG titers were enhanced by administering alum-precipitated protein, a modest additional protection was afforded. Therefore, despite SadA having pleiotropic functions, it is not a dominant, protective antigen for antibody-mediated protection against Salmonella.

Item Type: Article
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Date Deposited: 13 Oct 2015 13:14
Last Modified: 13 Oct 2015 13:14
URI: https://oak.novartis.com/id/eprint/6156

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