Feasibility and limits of magnetically labeling primary cultured rat T cells with ferumoxides coupled with commonly used transfection agents.
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Berger, Cedric, Rausch, Martin, Schmidt, Philipp and Rudin, Markus (2006) Feasibility and limits of magnetically labeling primary cultured rat T cells with ferumoxides coupled with commonly used transfection agents. Molecular Imaging : official journal of the Society for Molecular Imaging, 5 (2). pp. 93-104. ISSN 1535-3508
Abstract
Visualization and quantification of inflammatory processes is of high importance for early diagnosis of a multitude of diseases. Magnetic resonance imaging (MRI) using iron oxide (FeO) nanoparticles as contrast agents allows the study of macrophage infiltration during inflammation in a variety of tissues. Macrophages are effectors of the immune response, their appearance being orchestrated by activated T lymphocytes. Therefore, tracking of labeled T lymphocytes, which initiate the immune process, should enable earlier detection of tissue inflammation. In this study, we investigate the feasibility of specifically labeling harvested T cells by using dextran-coated FeO nanoparticles and commonly available transfection agents (TAs). Physicochemical properties of the newly formed FeO/TA vesicles were determined as well as their cell toxicity and their T cell activation potential. The labeling efficiency of each FeO/TA combination was evaluated by measuring the transverse MRI relaxation rate R(2) by X-ray spectroscopy and magnetic selection. Toxicity and labeling efficacy differed significantly among TAs. The best results were achieved by using polyamine TAs and in particular by using poly-l-lysine at a concentration of 1.5 microg/mL administered in combination with 22.5 microg iron/mL. By using this protocol, up to 60% of harvested T cells could be labeled. Microscopic investigation revealed FeO/TA nanoparticles not only localized within the cytoplasma of the cells but also sticking to the outer membrane surface.
| Item Type: | Article |
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| Additional Information: | author can archive post-print (ie final draft post-refereeing); 3 months for STM, 6 months for other titles, except Economics journals which are 12 months; Publisher's version/PDF must be used for post-print deposit; Publisher copyright and source must be acknowledged |
| Keywords: | T cell labeling; transfection agents; iron oxide particles; USPIO; MRI |
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| Date Deposited: | 14 Dec 2009 13:57 |
| Last Modified: | 31 Jan 2013 01:12 |
| URI: | https://oak.novartis.com/id/eprint/599 |