Michaut, Lydia and Engelhardt, Volker (2025) Master thesis proposal: Advancing non-invasive methods for evaluating therapeutic oligonucleotide efficacy in preclinical and clinical samples. University of Basel MSc student site. ISSN NA

Abstract

Novartis offers a project for a master’s student of the University of Basel interested in molecular biology and translational medicine focusing on novel therapeutic modalities.
Oligonucleotide therapeutics have emerged as precision medicines for previously untreatable diseases, by reducing gene expression through targeted mRNA cleavage. During drug development, defining the quantitative relationship between the dose and the extent of mRNA knockdown is pivotal to define a therapeutic window. Because oligonucleotide therapeutics act inside cells within specific organs, pharmacodynamic assessments often require tissue biopsies. Non-invasive approaches can reduce animal use and patient burden.
All cells release various types of extracellular vesicles (EVs) - including exosomes that carry nucleic acids, proteins, metabolites, and lipids reflecting the physiological state of their cells of origin. This enables liquid biopsy approaches that provide molecular information from tissues in a minimally invasive way.
This master’s thesis builds on proof-of-concept data validating the feasibility of approach and offers hands-on experience in advanced bioanalytics within the state-of-the-art laboratories of Pharmacokinetics Sciences. If you are interested in exosome isolation, multiplex digital PCR, transcriptome profiling, and ligand binding assays, and in enhancing your skills in experimental design, data analysis, and scientific communication, please contact us!

Item Type:	Article
Date Deposited:	03 Feb 2026 00:45
Last Modified:	03 Feb 2026 00:45
URI:	https://oak.novartis.com/id/eprint/59402