Poller, Birk and Walles, Markus (2024) Intestinal Cells-on-Chip for Permeability Studies. Intestinal Cells-on-Chip for Permeability Studies, 15 (12). pp. 1464-1469.

Abstract

Background: To accurately measure permeability of compounds in the intestine, here is a need for preclinical in vitro models that accurately represent the specificity, integrity and complexity of the human small intestinal barrier. Intestine-on-chip systems hold considerable promise as testing platforms, but several characteristics still require optimization and further development.
Methods: An established intestine-on chip model for tissue explants was here adopted for intes-tinal cell monolayer culture. Besides, membrane characteristics and standardized read-outs were further investigated.
Results: By starting cultures outside the chip in conventional wells plates, the new cell disc design could support accurate cell monolayer formation for both Caco-2 and human enteroids. When transferred to the chip with laminar flow, there was accurate detection of barrier integrity (FD4 and Cascade Blue) and permeability (atenolol/antipyrine).
Conclusions: This novel intestinal cell-on-chip system offers large flexibility for intestinal per-meability studies, although it still requires validation with more compounds to reveal its full potential

Item Type:	Article
Date Deposited:	17 Dec 2024 00:45
Last Modified:	17 Dec 2024 00:45
URI:	https://oak.novartis.com/id/eprint/55627