Grewe, Anja, Huesken, Dieter, Nitsche, Eric and Hildebrand, Kirsten (2011) Customization of the lysis and reaction setup steps of FastLane Cell kits for an automated workflow. QIAGEN News, 2011.

Abstract

Automated workflows can be optimized for enhanced flexibility by determining the steps within the protocol that tolerate extended storage of assay components. We determined the tolerated stopping points and storage conditions in a multiplex real-time RT-PCR workflow using the FastLane Cell Multiplex Kit. Breaks were introduced within the procedure and the effect of incubation times and temperatures on the observed CT values were measured. Using these parameters, we provide guidance on adapting the protocol to enable an automated workflow. We also suggest steps for achieving optimal results when using FastLane Cell kits with TaqMan Gene Expression Assays.

Item Type:	Article
Keywords:	siRNA, RT-QPCR
Date Deposited:	13 Oct 2015 13:15
Last Modified:	13 Oct 2015 13:15
URI:	https://oak.novartis.com/id/eprint/5538