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Light it up: Highly Efficient Multigene Delivery in Mammalian Cells

Trowitzsch, Simon, Klumpp, Martin, Thoma, Ralf, Carralot, Jean-Philippe and Berger, Imre (2011) Light it up: Highly Efficient Multigene Delivery in Mammalian Cells. Bioessays, 33 (12). pp. 946-955. ISSN 0265-9247


Multigene delivery and expression systems are emerging as key technologies for many applications in contemporary biology. We have developed new methods for multigene delivery and expression in eukaryotic hosts for a variety of applications, including production of protein complexes for structural biology and drug development, provision of multicomponent protein biologics and for cell-based assays. We implemented tandem recombineering to facilitate rapid generation of multicomponent gene expression constructs for efficient transformation of mammalian cells, resulting in homogenous cell populations. Analysis of multiple parameters in living cells may require co-expression of fluorescently tagged sensors simultaneously in a single cell, at defined and ideally controlled ratios. Our method enables such applications by overcoming currently limiting challenges.
Here, we review recent multigene delivery and expression strategies and their exploits in mammalian cells. We discuss applications in drug discovery assays, interaction studies and biologics production, which may benefit in the future from our novel approach.

Item Type: Article
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Additional Information: This is purely a review article and contains no Novartis data, compounds or targets whatsoever.
Keywords: multigene expression systems, baculovirus expression vector system, virus-like particles, high-throughput screening, high-content screening
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Date Deposited: 13 Oct 2015 13:15
Last Modified: 13 Oct 2015 13:15