Nguyen, Giang T, Xu, Shuying, Adams, Walter, Leong, John M, Bunnell, Stephen C, Mansour, Michael K, Sykes, David B and Mecsas, Joan (2021) Neutrophils require SKAP2 for reactive oxygen species production following C-type lectin and stimulation. iScience, 24 (8). p. 102871. ISSN 2589-0042

Abstract

Signaling cascades converting the recognition of pathogens to efficient inflammatory responses by neutrophils are critical for host survival. SKAP2, an adaptor protein, is required for reactive oxygen species (ROS) generation following neutrophil stimulation by integrins, formyl peptide receptors, and for host defense against the Gram-negative bacterial pathogens, and . Using neutrophils from murine HoxB8-immortalized progenitors, we show that SKAP2 in neutrophils is crucial for maximal ROS response to purified C-type lectin receptor agonists and to the fungal pathogens, and , and for robust killing of . Inside-out signaling to integrin and Syk phosphorylation occurred independently of SKAP2 after infection. However, Pyk2, ERK1/2, and p38 phosphorylation were significantly reduced after infection with and in neutrophils. These data demonstrate the importance of SKAP2 in ROS generation and host defense beyond antibacterial immunity to include CLRs and species

Item Type:	Article
Date Deposited:	19 Mar 2024 00:45
Last Modified:	19 Mar 2024 00:45
URI:	https://oak.novartis.com/id/eprint/53379