Ying, GuoQing, Zhu, FangFang, Yi, Yu, Chen, JianShu, Mei, JianFeng, Zhang, YanLu and Chen, ShuQing (2015) Selecting DNA aptamers for endotoxin separation. Biotechnology letters, 37 (8). pp. 1601-1605. ISSN 1573-6776

Abstract

OBJECTIVES

To select aptamers for endotoxin separation from a 75-nucleotide single-stranded DNA random library using systematic evolution of ligands by exponential enrichment.

RESULTS

After 15 rounds of selection, the final pool of aptamers was specific to endotoxin. Structural analysis of aptamers that appeared more than once suggested that one aptamer can form a G-quartet structure. Tests for binding affinity and specificity showed that this aptamer exhibited a high affinity for endotoxin. Using this aptamer, aptamer-magnetic beads were designed to separate endotoxin.

CONCLUSIONS

Using these aptamer-magnetic beads, a new method to separate endotoxin was developed to enable specific separation of endotoxin that can be applied to drug and food products.

Item Type:	Article
Date Deposited:	08 Nov 2023 00:45
Last Modified:	08 Nov 2023 00:45
URI:	https://oak.novartis.com/id/eprint/52233