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Evaluation of biomarker discovery approaches to detect protein biomarkers of acute renal allograft rejection.

Voshol, Johannes and Brendlen, Nathalie and Mueller, Dieter and Inverardi, Bruno and Augustin, Angelique and Pally, Charles and Wieczorek, Grazyna and Morris, Randall and Raulf, Friedrich and Van Oostrum, Jan (2005) Evaluation of biomarker discovery approaches to detect protein biomarkers of acute renal allograft rejection. Journal of Proteome Research, 4 (4). pp. 1192-1199. ISSN 1535-3893

Abstract

Management of host responses to allografts by immunosuppressive therapy is the cornerstone of transplantation medicine, but it is still deficient in one important element: biomarkers that are readily accessible and predict the fate of the transplant early, specifically, and reliably. Using a Brown Norway (BN)-to-Lewis rat renal allograft model of kidney transplantation, this study aims at evaluating two proteomic approaches to discover biomarkers for acute rejection: SELDI-MS technology and 2D gel electrophoresis combined with mass spectrometry. Several novel potential serum biomarkers have been identified for follow up. Overall, the conclusion is that apparently at the serum protein level, dramatic changes only occur at a stage where kidney function is already severely affected. Multivariate analysis of serum profiles suggests that there is an ensemble of subtle changes, comprising a proteomic signature of acute rejection at an early stage, a more detailed evaluation of which might provide novel opportunities for the diagnosis of acute rejection. Profiling of the excreted proteins indicates that urine might even present the earliest signs of the rejection process.

Item Type: Article
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Additional Information: archiving not formally supported by this publisher
Keywords: proteomics; acute rejection; biomarkers; transplantation; animal model; 2D-electrophoresis; SELDI−MS
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Date Deposited: 14 Dec 2009 13:58
Last Modified: 31 Jan 2013 01:14
URI: https://oak.novartis.com/id/eprint/517

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