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Development and validation of a generic fluorescent methyltransferase activity assay based on the Transcreener® AMP/GMP Assay

Klink, Tony A and Staeben, Matt and Twesten, Kim and Josvai, Rebecca and Kleman-Leyer, Karen and Klumpp, Martin and Lowery, Robert G (2011) Development and validation of a generic fluorescent methyltransferase activity assay based on the Transcreener® AMP/GMP Assay. Journal of Biomolecular Screening, 17 (1). pp. 59-70. ISSN 1087-0571

Abstract

Methylation is a ubiquitous covalent modification used to control the function of diverse biomolecules including hormones, neurotransmitters, xenobiotics, proteins, nucleic acids and lipids. Histone methyltransferases (HMTs) are currently of high interest as drug targets because of their role in epigenetic regulation, however most HMT assay methods are either not amenable to an HTS environment or are applicable to a limited number of enzymes. We developed a generic methyltransferase assay method using fluorescent immunodetection of AMP, which is formed from the MT reaction product S-adenosylhomocysteine in a dual enzyme coupling step. The detection range of the assay, its suitability for HTS, including stability of reagents following dispensing and after addition to reactions as well as the potential for interference from drug like molecules was investigated. In addition, the use of the assay for measuring inhibitor potencies with peptide or intact protein substrates was examined through pilot screening with selected reference enzymes including HMT G9a. By combining a novel enzymatic coupling step with the well characterized Transcreener® AMP/GMP assay, we have developed a robust HTS assay for HMTs which should be broadly applicable to other types of methyltransferases as well.

Item Type: Article
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Additional Information: author can archive post-print (ie final draft post-refereeing); Publisher's version/PDF cannot be used
Keywords: epigenetics, histone methyltransferase, methyltransferase assay
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Date Deposited: 13 Oct 2015 13:15
Last Modified: 13 Oct 2015 13:15
URI: https://oak.novartis.com/id/eprint/5114

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