Raghunathan, VijayKrishna (2024) Consensus Recommendations for ex vivo Anterior Segment Studies of Intraocular Pressure Facility and Regulation. Invest Ophthalmol Vis Sci ., 65 (14). p. 32.

Abstract

Intraocular pressure (IOP) elevation is the primary risk factor and currently only treatable parameter for progression of glaucomatous optic nerve damage. Since aqueous humor outflow regulation remains only partially understood at a basic science level and is the focus of much of clinical glaucoma activity, additional studies in these areas are of high importance. Aside from direct clinical studies, perfused anterior segment organ culture remains the primary model system for studying IOP regulation, although some in vitro animal models have been developed and are useful in certain cases. Perfused whole globes and stationary anterior segment organ culture provided the basis for perfused anterior segment organ culture. Although this approach can be applied to several other species, human tissue is the most common. Herein, we address the protocols for the most common methodological ex vivo human approaches, variations used in other species or in partial anterior segments and the viability and limitations of these approaches.

Item Type:	Article
Date Deposited:	21 Feb 2025 00:45
Last Modified:	21 Feb 2025 00:45
URI:	https://oak.novartis.com/id/eprint/50222