Liu, Samantha Y., Mulugeta, Naomi, Dougan, Stephanie K. and Qiang, Li (2023) In vitro flow cytometry assay to assess human and mouse macrophage phagocytosis of tumor cells. Cell. ISSN 1097-4172; 0092-8674

Abstract

Although tumor-associated macrophages are generally immunosuppressive, macrophages may also promote tumor clearance via phagocytosis of live tumor cells. Here, we describe a protocol for assessing macrophage engulfment of tumor cells in vitro using flow cytometry. The method is applicable to both mouse bone marrow-derived macrophages and human monocyte-derived macrophages. SMAC mimetics and recombinant IFN- or lymphotoxin are used as positive controls. This assay can be used to identify compounds affecting phagocytosis or to mechanistically interrogate pathways involved in phagocytic uptake.

Item Type:	Article
Date Deposited:	05 Jul 2023 00:45
Last Modified:	05 Jul 2023 00:45
URI:	https://oak.novartis.com/id/eprint/49488