Mueller, Matthias and Fruh, Isabelle (2023) Engineering circuits of human iPSC-derived neurons and rat primary glia. Frontiers in neuroscience, 17. ISSN 1662-4548

Abstract

Novel in vitro platforms based on human neurons are needed to improve early drug testing and address the stalling drug discovery in neurological disorders. Topologically controlled circuits of human induced pluripotent stem cell (iPSC)-derived neurons have the potential to become such a testing system. In this work, we build in vitro co-cultured circuits of human iPSC-derived neurons and rat primary glial cells using microfabricated polydimethylsiloxane (PDMS) structures on microelectrode arrays (MEAs). The circuits are achieved by seeding different neuron-to glia ratios either as dissociated cells or pre-aggregated spheroids. An antifouling coating is developed to prevent axonal overgrowth in undesired locations of the microstructure. We assess the electrophysiological properties of different types of circuits over more than 50 days, including their stimulation-induced neural activity. Finally, as a proof-of-concept for screening of activity altering compounds, we demonstrate the effect of magnesium chloride on the electrical activity of circuits.

Item Type:	Article
Date Deposited:	27 Jun 2023 00:46
Last Modified:	27 Jun 2023 00:46
URI:	https://oak.novartis.com/id/eprint/48970