Harwood, Matthew D, Zettl, Katharina, Weinheimer, Manuel, Pilla-Reddy, Venkatesh, Shen, Hong, Jacobs, Frank, Chu, Xiaoyan, Huth, Felix, Nakakariya, Masanori, Chothe, Paresh, Neuhoff, Sibylle and Wisniewski, Jacek R (2023) Interlaboratory Variability in the Madin-Darby Canine Kidney Cell Proteome. Molecular pharmaceutics. ISSN 1543-8392

Abstract

Madin-Darby canine kidney (MDCK) cells are widely used to study epithelial cell functionality. Their low endogenous drug transporter protein levels make them an amenable system to investigate transepithelial permeation and drug transporter protein activity after their transfection. MDCK cells display diverse phenotypic traits, and as such, laboratory-to-laboratory variability in drug permeability assessments is observed. Consequently, in vitro-in vivo extrapolation (IVIVE) approaches using permeability and/or transporter activity data require calibration. A comprehensive proteomic quantification of 11 filter-grown parental or mock-transfected MDCK monolayers from 8 different pharmaceutical laboratories using the total protein approach (TPA) is provided. The TPA enables estimations of key morphometric parameters such as monolayer cellularity and volume. Overall, metabolic liability to xenobiotics is likely to be limited for MDCK cells due to the low expression of required enzymes. SLC16A1 (MCT1) was the highest abundant SLC transporter linked to xenobiotic activity, while ABCC4 (MRP4) was the highest abundant ABC transporter. Our data supports existing findings that claudin-2 levels may be linked to tight junction modulation, thus impacting trans-epithelial resistance. This unique database provides data on more than 8000 protein copy numbers and concentrations, thus allowing an in-depth appraisal of the control monolayers used in each laboratory.

Item Type:	Article
Date Deposited:	27 Jun 2023 00:46
Last Modified:	27 Jun 2023 00:46
URI:	https://oak.novartis.com/id/eprint/48233