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Electrical Impedance as a Novel Biomarker of Myotube Atrophy and Hypertrophy

Rakhilin, Sergey and Turner, Gordon and Katz, Mark and Warden, Robert and Irelan, Jeffrey and Abassi, Yama and Glass, David (2011) Electrical Impedance as a Novel Biomarker of Myotube Atrophy and Hypertrophy. Journal of Biomolecular Screening. ISSN 1087-0571

Abstract

Measuring myotube thickness is the most physiological and unbiased approach for screening therapeutic compounds that prevent skeletal muscle atrophy or induce hypertrophy. However, an accurate cell thickness estimate is often quite challenging due to the extreme heterogeneity of the myotube cellular population and the relatively small changes in cell diameter caused by either atrophy or hypertrophy.
Here we present two novel methods to evaluate changes in myotube thickness including a computer-based image analysis program and a measurement of cellular electrical impedance. The first approach represents an Image J-based program that estimates mean thickness of all myotubes identified via microscopy based upon gluteraldehyde-induced auto fluorescence. The second approach measures changes in electrical current impedance as a function of cellular adhesion in real time that can be used as a biomarker for myotube atrophy or hypertrophy. Application of these techniques to drug screening might be beneficial in finding novel treatments preventing muscle atrophy and other diseases associated with any morphological change in cell shape.

Item Type: Article
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Additional Information: author can archive post-print (ie final draft post-refereeing); Publisher's version/PDF cannot be used
Keywords: dexamethasone - dex Phosphate buffer saline -PBS Insulin growth Factor 1 - IGF-1
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Date Deposited: 13 Oct 2015 13:15
Last Modified: 13 Oct 2015 13:15
URI: https://oak.novartis.com/id/eprint/4805

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