Lopez, John, Ackermann, Damian and Heilmann, Tobias (2022) Refractive Index to Monitor Solid-Phase Oligonucleotide Synthesis. Organic Process Research & Development. ISSN 1083-61601520-586X

Abstract

Solid-phase oligonucleotide synthesis is a mature and efficient automated process, which is used in academia and industry since decades. However, some steps are still run in a black box modus and only a few process analytical technology tools for in-process control are available, namely UV-Vis spectroscopy and conductivity. In order to ensure high yields in each of the numerous steps, the process is usually performed using an excess of starting materials, reagents, solvents. The reaction times are defined according to standard procedures and not the real need of the reaction.. In consequence, Solid-phase oligonucleotide synthesis suffers from generating large amounts of toxic waste and it is a challenge from the sustainability perspective.. One approach to make more sustainable that process is to use add more complementary monitoring tools that provide online information about the progress of the process. Herein, we disclose our promising preliminary results in the use of refractive index to monitor solid phase oligonucleotide synthesis.

Item Type:	Article
Date Deposited:	30 Dec 2022 00:45
Last Modified:	30 Dec 2022 00:45
URI:	https://oak.novartis.com/id/eprint/47773