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Differentiation and visualization of diverse cellular phenotypic responses in primary high-content screening

Kuemmel, Anne and Selzer, Paul and Siebert, Daniela and Schmidt, Isabel and Reinhardt, Juergen and Goette, Marjo and Ibig-Rehm, Yvonne and Parker, Christian and Gabriel, Daniela (2011) Differentiation and visualization of diverse cellular phenotypic responses in primary high-content screening. Journal of Biomolecular Screening. ISSN 1087-0571

Abstract

High throughput screening based on sub-cellular imaging has become a powerful tool in lead discovery. Through the generation of high quality images not only the specific target signal can be analyzed but also phenotypic changes of the whole cell are recorded. Yet analysis strategies for the exploration of high content screening results in a manner that is independent on predefined control phenotypes are largely missing. The approach presented here is based on a modeling technique, self-organizing maps (SOMs), which utilizes multi-parametric results by grouping treatments that create similar morphological effects. As a new way to visualize the results each node is represented with an image highlighting the representative cell phenotype. The approach has the potential to identify both expected hits as well as novel cell phenotypes. Moreover, different compound chemotypes, which cause the same phenotypic effects, are identified facilitating “scaffold hopping”.

Item Type: Article
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Additional Information: author can archive post-print (ie final draft post-refereeing); Publisher's version/PDF cannot be used
Keywords: high content screening, multi-parametric data analysis, SOM
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Date Deposited: 13 Oct 2015 13:15
Last Modified: 13 Oct 2015 13:15
URI: https://oak.novartis.com/id/eprint/4707

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