Carmen, Pessuti, Medley, Quintus, Li, Ning, Huang, Chia-Ling, Loureiro, Joe, Banks, Angela, Zhang, Qin, Costa, Deise, Ribeiro, Kleber, Nascimento, Heloisa, Muccioli, Cristina, Commodaro, Alessandra, Huang, Qian and Belfort, Rubens (2023) Differential proteins expression distinguished between patients with infectious and noninfectious uveitis. Ocular Immunology & Inflammation. ISSN 0927-3948 , 1744-5078

Abstract

Uveitis is associated with a number of inflammatory diseases and can be infectious or noninfectious. The diagnosis of uveitis is usually based on typical clinical presentation, but sometimes the signs and symptoms are not clear, and the discovery of new biomarkers may help to distinguish between infectious and noninfectious uveitis. In the current study, we investigate the proteome of aqueous humor and associated blood plasma from patients with confirmed infectious or noninfectious uveitis. Aqueous humor (AH) and plasma were obtained from 28 patients with infectious uveitis (IU), 29 patients with noninfectious uveitis (NIU) and 35 healthy controls undergoing cataract surgery. The protein profiles were analyzed by SomaScan proteomic profiling technology. Our results showed proteins differentially expressed in the AH and plasma from IU and NIU patients in comparison with cataract patients. Importantly, 18 proteins were up-regulated and 1 protein was down-regulated in both AH and plasma from IU patients. Furthermore, in NIU patients, 142 proteins were up-regulated and 7 proteins down-regulated in both AH and blood plasma. The results of pathway enrichment analysis for both IU and NIU groups identified mostly to inflammatory and regulatory processes. In conclusion, SomaScan assay was able to detect novel AH and plasma protein biomarkers in IU and NIU patients. The unique proteins found in both AH and plasma suggests a protein signature that could distinguish between infectious and noninfectious uveitis patient populations.

Item Type:	Article
Keywords:	SOMAscan, infectious uveitis, noninfectious uveitis, proteomic platform, biomarkers
Date Deposited:	14 Apr 2023 00:46
Last Modified:	14 Apr 2023 00:46
URI:	https://oak.novartis.com/id/eprint/46863