Browse views: by Year, by Function, by GLF, by Subfunction, by Conference, by Journal

Multiplexed Reporter Gene Assays: Monitoring the cell viability and the compound kinetics on luciferase activity

Didiot, Marie-Cecile and Serafini, Sergio and Pfeifer, Martin and King, Frederick and Parker, Christian (2011) Multiplexed Reporter Gene Assays: Monitoring the cell viability and the compound kinetics on luciferase activity. Journal of Biomolecular Screening, 16 (7). pp. 786-793. ISSN 1087-0571

Abstract

High throughput screening assays with multiple readouts enable one to monitor multiple assay parameters. By capturing as much information about the underlying biology as possible the detection of true actives can be improved. This report describes an extension to standard luciferase reporter gene assays that enables multiple parameters to be monitored from each sample. The report describes multiplexing luciferase assays with an orthogonal read-out monitoring cell viability using reduction of resazurin. In addition, this technical note shows that by using the luciferin substrate in live cells an assay time course can be recorded. This enables the identification of non-active or unspecific compounds that act by inhibiting luciferase, as well as compounds altering gene expression or cell growth.

Item Type: Article
Related URLs:
Additional Information: author can archive post-print (ie final draft post-refereeing); Publisher's version/PDF cannot be used
Related URLs:
Date Deposited: 13 Oct 2015 13:15
Last Modified: 13 Oct 2015 13:15
URI: https://oak.novartis.com/id/eprint/4449

Search

Email Alerts

Register with OAK to receive email alerts for saved searches.