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EndoBind - Real-time detection of endogenous protein-protein interactions

Bill, Anke, Espinola, Sheryll, Guthy, Daniel Alexander, Lu, Min, Marelli, Anthony, Mendiola, Angelica, Miraglia, Loren, Taylor, Brandon, Haling, Jake, Vargas, Leonardo, Orth, Anthony, King, Frederick and Lanter, Mylene (2021) EndoBind - Real-time detection of endogenous protein-protein interactions. Nature communications Biology.


We present two methods to detect the interaction of ectopically expressed (RT-Bind) or endogenously tagged (EndoBind) proteins of interest. Both approaches provide temporal evaluation of dimer over an extended duration. Using examples of the NRF2-KEAP1 and the CRAF-KRAS_G12V interaction, we demonstrate that our method allows for the detection of signal for more than 2 days after substrate addition, allowing for continuous monitoring of the protein-protein interaction in real time.

Item Type: Article
Date Deposited: 12 Oct 2021 00:45
Last Modified: 12 Oct 2021 00:45


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