Bill, Anke, Espinola, Sheryll, Guthy, Daniel Alexander, Lu, Min, Marelli, Anthony, Mendiola, Angelica, Miraglia, Loren, Taylor, Brandon, Haling, Jake, Vargas, Leonardo, Orth, Anthony, King, Frederick and Lanter, Mylene (2021) EndoBind - Real-time detection of endogenous protein-protein interactions. Nature communications Biology.

Abstract

We present two methods to detect the interaction of ectopically expressed (RT-Bind) or endogenously tagged (EndoBind) proteins of interest. Both approaches provide temporal evaluation of dimer over an extended duration. Using examples of the NRF2-KEAP1 and the CRAF-KRAS_G12V interaction, we demonstrate that our method allows for the detection of signal for more than 2 days after substrate addition, allowing for continuous monitoring of the protein-protein interaction in real time.

Item Type:	Article
Date Deposited:	12 Oct 2021 00:45
Last Modified:	12 Oct 2021 00:45
URI:	https://oak.novartis.com/id/eprint/43994