Lee, Jee and Kemp, Daniel (2006) Human adipose-derived stem cells display myogenic potential and perturbed function in hypoxic conditions. Biochemical and Biophysical Research Communications, 341 (3). pp. 882-888. ISSN 0006-291X

Abstract

Here, we enriched a human cell population from adipose tissue that exhibited both mesenchymal plasticity, self-renewal capacity, and a cell-surface marker profile indistinguishable from that of bone marrow-derived mesenchymal stem cells. In addition to adipogenic and osteogenic differentiation, these adipose-derived stem cells displayed skeletal myogenic potential when co-cultured with mouse skeletal myocytes in reduced serum conditions. Physical incorporation of stem cells into multinucleated skeletal myotubes was determined by genetic lineage tracing, whereas human-specific antibody staining was employed to demonstrate functional contribution of the stem cells to a myogenic lineage. To investigate the effects of hypoxia, cells were maintained and differentiated at 2% O(2). In contrast with reports on bone marrow-derived stem cells, both osteogenic and adipogenic differentiation were significantly attenuated. In summary, the relative accessibility of adipose-derived mesenchymal stem cells from human donors provides opportunity for molecular investigation of mechanistic dysfunction in disease settings and may introduce new prospects for cell-based therapy.

Item Type:	Article
Related URLs:	http://www.ncbi.nlm.nih.gov/sites/entrez... doi:10.1016/j.bbrc.2006.01.038
Additional Information:	author can archive post-print (ie final draft post-refereeing); Publisher's version/PDF cannot be used
Keywords:	Mesenchymal; Stem cells; Osteogenesis; Adipogenesis; Myogenesis; Hypoxia; Differentiation; Plasticity
Related URLs:	http://www.ncbi.nlm.nih.gov/sites/entrez... doi:10.1016/j.bbrc.2006.01.038
Date Deposited:	14 Dec 2009 14:00
Last Modified:	14 Dec 2009 14:00
URI:	https://oak.novartis.com/id/eprint/434