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A practical approach to address the issue of analyte nonspecific binding in quantitative analysis of untreated and transferred urine samples

Fu, Yunlin and Li, Wenkui and Smith, Harold and Tse, Francis (2011) A practical approach to address the issue of analyte nonspecific binding in quantitative analysis of untreated and transferred urine samples. Bioanalysis, 3 (9). pp. 967-972. ISSN 1757-6180

Abstract

Background:
Urine samples often are collected and transferred before a small degree of nonspecific binding (NSB) was noticed for the analyte of interest in quantitative analysis.

Results:
In a case study for the determination of compound A in human urine, an approach taken to address the issue was to (1) use STDs and QCs to mimic the study samples and (2) to use Tween-80 (0.5%) to retrieve the adsorbed analyte. The method was validated in pre-study validation. However, incurred sample reanalysis (ISR) failed. Investigation unveiled incomplete retrieval of the adsorbed analyte and non-homogeneity of the study samples as the cause of assay failure. All samples were reanalyzed using a modified sample mixing method, which resulted in two successful ISR runs.

Conclusions:
Untreated and transferred urine samples can be accurately analyzed by using the STDs and QCs to mimic study samples and employing a surfactant to retrieve the adsorbed analyte. Thorough sample mixing is important to ensure accurate and reproducible analysis.

Item Type: Article
Related URLs:
Additional Information: Publisher's version/PDF cannot be used (unless Paid Option used
Keywords: Urine assay, LC-MS/MS, Nonspecific binding (NSB), Incurred sample reanalysis, Vortex-mixer, Rotary-mixer
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Date Deposited: 13 Oct 2015 13:15
Last Modified: 13 Oct 2015 13:15
URI: https://oak.novartis.com/id/eprint/4296

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