A practical approach to address the issue of analyte nonspecific binding in quantitative analysis of untreated and transferred urine samples
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Fu, Yunlin, Li, Wenkui, Smith, Harold and Tse, Francis (2011) A practical approach to address the issue of analyte nonspecific binding in quantitative analysis of untreated and transferred urine samples. Bioanalysis, 3 (9). pp. 967-972. ISSN 1757-6180
Abstract
Background:
Urine samples often are collected and transferred before a small degree of nonspecific binding (NSB) was noticed for the analyte of interest in quantitative analysis.
Results:
In a case study for the determination of compound A in human urine, an approach taken to address the issue was to (1) use STDs and QCs to mimic the study samples and (2) to use Tween-80 (0.5%) to retrieve the adsorbed analyte. The method was validated in pre-study validation. However, incurred sample reanalysis (ISR) failed. Investigation unveiled incomplete retrieval of the adsorbed analyte and non-homogeneity of the study samples as the cause of assay failure. All samples were reanalyzed using a modified sample mixing method, which resulted in two successful ISR runs.
Conclusions:
Untreated and transferred urine samples can be accurately analyzed by using the STDs and QCs to mimic study samples and employing a surfactant to retrieve the adsorbed analyte. Thorough sample mixing is important to ensure accurate and reproducible analysis.
| Item Type: | Article |
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| Additional Information: | Publisher's version/PDF cannot be used (unless Paid Option used |
| Keywords: | Urine assay, LC-MS/MS, Nonspecific binding (NSB), Incurred sample reanalysis, Vortex-mixer, Rotary-mixer |
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| Date Deposited: | 13 Oct 2015 13:15 |
| Last Modified: | 13 Oct 2015 13:15 |
| URI: | https://oak.novartis.com/id/eprint/4296 |