Iurlaro, Mario, Stadler, Michael, Galli, Giorgio, Jagani, Zainab and Schuebeler, Dirk (2021) Mammalian SWI/SNF continuously restores local accessibility to chromatin. Nature Genetics. ISSN 15461718

Abstract

Chromatin accessibility is a hallmark of regulatory regions, entails transcription factor (TF) binding and requires nucleosomal reorganization. However, it remains unclear how dynamic this process is. In the present study, we use small-molecule inhibition of the catalytic subunit of the mouse SWI/SNF remodeler complex to show that accessibility and reduced nucleosome presence at TF-binding sites rely on persistent activity of nucleosome remodelers. Within minutes of remodeler inhibition, accessibility and TF binding decrease. Although this is irrespective of TF function, we show that the activating TF OCT4 (POU5F1) exhibits a faster response than the repressive TF REST. Accessibility, nucleosome depletion and gene expression are rapidly restored on inhibitor removal, suggesting that accessible chromatin is regenerated continuously and in a largely cell-autonomous fashion. We postulate that TF binding to chromatin and remodeler-mediated nucleosomal removal do not represent a stable situation, but instead accessible chromatin reflects an average of a dynamic process under continued renewal.

Item Type:	Article
Date Deposited:	14 May 2021 00:45
Last Modified:	14 May 2021 00:45
URI:	https://oak.novartis.com/id/eprint/42563