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Development of a biochemical and biophysical suite for integral membrane protein targets: A review

Haffke, Matthias and Duckely, Myriam and Bergsdorf, Christian and Jaakola, Veli-Pekka and Shrestha, Binesh (2019) Development of a biochemical and biophysical suite for integral membrane protein targets: A review. Protein expression and purification. ISSN 10960279

Abstract

The generation of integral membrane proteins (IMPs) in heterologous systems and their characterization remains a major challenge in biomedical research. Significant efforts have been invested both in academia and in the pharmaceutical industry to establish technologies for the expression, isolation and characterization of IMPs. Here we summarize some of the key aspects, which are important to support structure-based drug design (SBDD) in drug discovery projects. We furthermore include timeline estimates and an overview of the target selection and biophysical screening approaches.

Item Type: Article
Keywords: Expression G protein coupled receptors GPCR Insect cells Integral membrane proteins Mammalian cells
Date Deposited: 20 Dec 2019 00:45
Last Modified: 20 Dec 2019 00:45
URI: https://oak.novartis.com/id/eprint/41684

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