Jolly, Christopher J. , Lee, Quintin, Padula,, Matthew P. , Pinello, Natalia, O'Rourke, Matthew B. , Williams, Simon , de Souza, William Marciel , Orkin, Joseph, Melin, Amanda, Shaban, Babak, Weninger, Wolfgang, Wong, Justin J.-L. , Crim, Marcus, Monette , Sébastien, Roediger, Ben, Fumagalli, Marcilio Jorge, Song, Renhua, Brenner, Ori and Pimanda, John E. (2020) Murine and related chapparvoviruses are nephro-tropic and produce novel accessory proteins in infected kidneys. Murine and related chapparvoviruses are nephro-tropic and produce novel accessory proteins in infected kidneys, 16 (1). ISSN 1553-7366

Abstract

Mouse kidney parvovirus (MKPV) is a member of the provisional genus Chapparvovirus that causes renal disease in immune-compromised mice, with a disease course reminiscent of polyomavirus-associated nephropathy in immune-suppressed kidney transplant patients. Here we map four major MKPV transcripts, created by alternative splicing, to a common initiator region, and use mass spectrometry to identify "p10" and "p15" as novel chapparvovirus accessory proteins produced in MKPV-infected kidneys. p15 and the splicing-dependent putative accessory protein NS2 are conserved in all near-complete amniote chapparvovirus genomes currently available (from mammals, birds and a reptile). In contrast, p10 may be encoded only by viruses with >60% amino acid identity to MKPV. We show that MKPV is kidney-tropic and that the bat chapparvovirus DrPV-1 and a non-human primate chapparvovirus, CKPV, are also found in the kidneys of their hosts. We propose, therefore, that many mammal chapparvoviruses are likely to be nephrotropic.

Item Type:	Article
Keywords:	Mouse kidney parvovirus
Date Deposited:	17 Mar 2020 00:45
Last Modified:	17 Mar 2020 00:45
URI:	https://oak.novartis.com/id/eprint/40619