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Genetic Reactivation of Cone Photoreceptors Restores Visual Responses in Retinitis Pigmentosa

Busskamp, Volker and Duebel, Jens and Balya, David and Fradot, Mathias and Viney, Tim James and Siegert, Sandra and Groner, Anna C and Cabuy, Erik and Forster, Valérie and Seeliger, Mathias and Biel, Martin and Humphries, Peter and Paques, Michel and Mohand-Said, Saddek and Trono, Didier and Deisseroth, Karl and Sahel, José A and Picaud, Serge and Roska, Botond (2010) Genetic Reactivation of Cone Photoreceptors Restores Visual Responses in Retinitis Pigmentosa. Science (New York, N.Y.), 329 (5990). pp. 413-417. ISSN 1095-9203

Abstract

Retinitis pigmentosa refers to a diverse group of hereditary diseases that lead to incurable blindness, affecting two million people worldwide. As a common pathology, rod photoreceptors die early, whereas light-insensitive, morphologically altered cone photoreceptors persist longer. It is unknown if these cones are accessible for therapeutic intervention. Here, we show that expression of archaebacterial halorhodopsin in light-insensitive cones can substitute for the native phototransduction cascade and restore light sensitivity in mouse models of retinitis pigmentosa. Resensitized photoreceptors activate all retinal cone pathways, drive sophisticated retinal circuit functions (including directional selectivity), activate cortical circuits, and mediate visually guided behaviors. Using human ex vivo retinas, we show that halorhodopsin can reactivate light-insensitive human photoreceptors. Finally, we identified blind patients with persisting, light-insensitive cones for potential halorhodopsin-based therapy.

Item Type: Article
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Additional Information: author can archive post-print (ie final draft post-refereeing); On author's personal web site
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Date Deposited: 04 Dec 2010 00:45
Last Modified: 01 Feb 2013 00:48
URI: https://oak.novartis.com/id/eprint/3967

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