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LARP-1 promotes oogenesis by repressing fem-3 in the C. elegans germline.

Zanin, Esther and Pacquelet, Anne and Scheckel, Claudia and Ciosk, Rafal and Gotta, Monica (2010) LARP-1 promotes oogenesis by repressing fem-3 in the C. elegans germline. Journal of Cell Science, 123 (Pt 16). pp. 2717-2724. ISSN 1477-9137

Abstract

LA-related protein 1 (LARP-1) belongs to an RNA-binding protein family containing a LA motif. Here, we identify LARP-1 as a regulator of sex determination. In C. elegans hermaphrodites, a complex regulatory network regulates the switch from sperm to oocyte production. We find that simultaneous depletion of larp-1 and the Nanos homologue nos-3 results in germline masculinization. This phenotype is accompanied by a strong reduction of the levels of TRA-1, a GLI-family transcription factor that promotes oogenesis. TRA-1 levels are regulated by CBC(FEM-1), a ubiquitin ligase consisting of the FEM proteins, FEM-1, FEM-2 and FEM-3 and the cullin CUL-2. We show that both the masculinization phenotype and the reduction of TRA-1 levels observed in nos-3;larp-1 mutants require fem-3 activity, suggesting that nos-3 and larp-1 regulate the sperm-oocyte switch by inhibiting the fem genes. Consistently, fem-3 mRNA levels are increased in larp-1 mutants. By contrast, levels of fem-3 mRNA are not affected in nos-3 mutants. Therefore, our data indicate that LARP-1 and NOS-3 promote oogenesis by regulating fem-3 expression through distinct mechanisms.

Item Type: Article
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Additional Information: author can archive post-print (ie final draft post-refereeing); On author's personal web site; Publisher's version/PDF may be used
Keywords: Sex determination, LARP-1, TRA-1, NOS-3, La-motif, Nanos, FEM proteins, Caenorhabditis elegans
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Date Deposited: 02 Nov 2010 00:45
Last Modified: 01 Feb 2013 00:48
URI: https://oak.novartis.com/id/eprint/3910

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