Renner, Magdalena, Cowan, Cameron, Gross-Scherf, Brigitte, Goldblum, David, Krol, Jacek, Szikra, Tamas, Papasaikas, Panagiotus, Munz, Martin, Cuttat-Theurillat, Rachel, Waldt, Annick, Diggelmann, Roland, Patino-Alvarez, Claudia, Gerber-Hollbach, Nadine, Schuierer, Sven, Srdanovic, Aldin, Balogh, Marton, Pascal, Hasler, Kusnyerik, Akos, Szabo, Arnold, Stadler, Michael, Hierlemann, Andreas, Scholl, Hendrik, Roma, Guglielmo, Nigsch, Florian and Roska, Botond Cell types of the human retina and its organoids at single cell resolution: developmental convergence, transcriptional identity, and disease map.

Abstract

Organoids are stem cell-derived artificial organs that mimic aspects of organ development, function and disease. How closely cell type diversity and cell type maturation in human organoids recapitulate that of their target organ is not well understood. We performed histochemistry and sequenced the RNA of 163,971 single cells from improved human retinal organoids at different developmental stages and from donated healthy adult human retinas and choroid. Cell types in mature organoids had morphologies and transcriptomes that resembled their adult equivalents. Remarkably, organoids developed at a similar rate to the developing human retina and the transcriptome trajectory of cell types contained a progression of key developmental markers. Mapping disease-associated genes to cell types revealed cellular targets for studying disease mechanism in organoids and performing targeted repair in adult retinas.

Item Type:	Article
Date Deposited:	16 May 2019 00:45
Last Modified:	16 May 2019 00:45
URI:	https://oak.novartis.com/id/eprint/38908