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Bleomycin-induced lung injury assessed noninvasively and in spontaneously breathing rats by proton MRI.

Karmouty Quintana, Harry and Cannet, Catherine and Zurbruegg, Stefan and Ble, Francois-Xavier and Fozard, John R. and Page, Clive and Beckmann, Nicolau (2007) Bleomycin-induced lung injury assessed noninvasively and in spontaneously breathing rats by proton MRI. Journal of Magnetic Resonance Imaging: JMRI, 26 (4). pp. 941-949. ISSN 1053-1807

Abstract

PURPOSE: To apply proton magnetic resonance imaging (MRI) techniques to assess noninvasively and in spontaneously breathing rats, structural changes following a single intratracheal administration of bleomycin (BLM). MATERIALS AND METHODS: Rats were scanned by MRI prior to BLM or vehicle administration and at six hours, 24 hours, week 1, and at weeks 2, 3, 6, and 8 after treatment. Bronchoalveolar lavage (BAL) fluid and histological analyses were performed at 24 hours, and at weeks 1 and 8 (histology only). RESULTS: Prominent MRI fluid signals were detected in the lungs of BLM-treated rats one week after challenge. These signals correlated with increased inflammatory parameters in BAL fluid and with marked perivascular and parenchymal infiltration with inflammatory cells in histological slices. At week 2 the MRI signals due to edema resolved, but nevertheless an increase in MRI signal intensity from the lung parenchyma was apparent. In some areas of the right lung the MRI signal intensity in the parenchyma decreased between weeks 2 and 8. These observations were in line with histology demonstrating collagen deposition and atelectasis (hallmarks of fibrosis) at week 1 and a partial recovery of the lung parenchyma at week 8. CONCLUSION: The data demonstrate the ability of proton MRI to detect BLM-induced lung fibrosis as well as the acute inflammatory response caused by the agent.

Item Type: Article
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Additional Information: archiving not allowed on institutional repository
Keywords: atelectasis; inflammation; lung; imaging; magnetic resonance imaging; bleomycin; fibrosis
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Date Deposited: 14 Dec 2009 14:01
Last Modified: 31 Jan 2013 01:18
URI: https://oak.novartis.com/id/eprint/370

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