Rue, Sarah, Anderson, Paul, Miller, Jessica, Fanale, Salvatore, Chang, Jim, Glaser, Scott and Lesley, Scott (2018) Mammalian cell culture density determination using a laser thru-beam sensor. BioTechniques, 65 (4). pp. 224-226. ISSN btn-2018-0059

Abstract

High-throughput protein expression platforms are increasingly used to produce proteins for many applications: to support studies in structure/function, regulation, and proteomics, as well as for direct use as potential biotherapeutic agents for medical applications. Here we describe a modification to the automated protein expression platform used at GNF in which a spectrophotometric sensor and a customized culture flask-receiving nest have been combined in a device that we refer to as the flask density reader (FDR). The FDR enables rapid, non-invasive, automated spectrophotometric determination of cell densities. The FDR reduces the risk of contamination with frequent flask sampling and greatly reduces the time and effort needed to count cells using off-line methods.

Item Type:	Article
Keywords:	automation cell counting cell culture cell density laser mammalian spectrophotometry
Date Deposited:	08 Dec 2018 00:45
Last Modified:	08 Dec 2018 00:45
URI:	https://oak.novartis.com/id/eprint/36071