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3′-Modification stabilizes mRNA and increases translation in cells

Gampe, Christian, White, Amy, Siva, Swetha, Zecri, Frederic and Diener, John (2018) 3′-Modification stabilizes mRNA and increases translation in cells. Bioorganic and Medicinal Chemistry Letters. ISSN 1464-3405


Successful implementation of mRNA gene therapy is facing many hurdles, for example poor expression levels of the exogenously delivered mRNA transcripts. Herein we describe the synthesis of various 3′-modified RNA oligonucleotides, and we show that 3′-modification drastically stabilizes these oligonucleotides in cell extracts. Modification of the 3′-terminus of gaussia luciferase mRNA results in 3-fold increased and extended (>48 h) translation of the mRNA. Our findings suggest 3′-modification of RNA-transcripts as a valid approach to increase expression levels for application in mRNA gene therapy.

Item Type: Article
Keywords: 3′-Terminus Bioconjugation Gene therapy mRNA Nucleotide Translation
Date Deposited: 04 Jul 2018 00:45
Last Modified: 25 Jan 2019 00:45


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