Browse views: by Year, by Function, by GLF, by Subfunction, by Conference, by Journal

Tricyclic antidepressants inhibit hippocampal α7*and α9α10 nicotinic acetylcholine receptors by different mechanisms

Arias, HR and Vázquez-Gómez, E and Hernández-Abrego, A and Gallino, S and Feuerbach, Dominik and Ortells, MO and Elgoyhen, AB and García-Colunga, J (2018) Tricyclic antidepressants inhibit hippocampal α7*and α9α10 nicotinic acetylcholine receptors by different mechanisms. The international journal of biochemistry & cell biology.

Abstract

The activity of tricyclic antidepressants (TCAs) at α7 and α9α10 nicotinic acetylcholine receptors (AChRs) as well as at hippocampal α7-containing (i.e., α7*) AChRs is determined by using Ca2+ influx and electrophysiological recordings. To determine the inhibitory mechanisms, additional functional tests and molecular docking experiments are performed. The results established that TCAs (a) inhibit Ca2+ influx in GH3-α7 cells with the following potency (IC50 in µM) rank: amitriptyline (2.7 ± 0.3) > doxepin (5.9 ± 1.1) ~ imipramine (6.6 ± 1.0). Interestingly, imipramine inhibits hippocampal α7* AChRs (42.2 ± 8.5 μM) in a noncompetitive and voltage-dependent manner, whereas it inhibits α9α10 AChRs (0.53 ± 0.05 µM) in a competitive and voltage-independent manner, and (b) inhibit [3H]imipramine binding to resting α7 AChRs with the following affinity rank (IC50 in μM): imipramine (1.6 ± 0.2) > amitriptyline (2.4 ± 0.3) > doxepin (4.9± 0.6), whereas imipramine’s affinity was no significantly different to that for the desensitized state. The molecular docking and functional results support the notion that imipramine noncompetitively inhibits α7 AChRs by interacting with two overlapping luminal sites, whereas it competitively inhibits α9α10 AChRs by interacting with the orthosteric sites. Collectively our data indicate that TCAs inhibit α7, α9α10, and hippocampal α7* AChRs at clinically relevant concentrations and by different mechanisms of action.

Item Type: Article
Date Deposited: 18 May 2018 00:45
Last Modified: 18 May 2018 00:45
URI: https://oak.novartis.com/id/eprint/35628

Search

Email Alerts

Register with OAK to receive email alerts for saved searches.