Browse views: by Year, by Function, by GLF, by Subfunction, by Conference, by Journal

A Scalable Pipeline for High-Throughput Flow Cytometry

Wilson, Aaron and Moutsatsos, Ioannis and Pineda, Javier and Feng, Yan and Yu, Gary and Auld, Douglas (2018) A Scalable Pipeline for High-Throughput Flow Cytometry. SLAS Discovery. ISSN 24725560

Abstract

Flow cytometry (FC) provides high-content data for a variety of applications, including phenotypic analysis of cell surface and intracellular markers, characterization of cell supernatant or lysates, and gene expression analysis. Historically, sample preparation, acquisition, and analysis have presented as a bottleneck for running such types of assays at scale. This article will outline the solutions that have been implemented at Novartis which have allowed high-throughput FC to be successfully conducted and analyzed for a variety of cell-based assays. While these experiments were generally conducted to measure phenotypic responses from a well-characterized and information-rich small molecular probe library known as the Mechanism-of-Action (MoA) Box, they are broadly applicable to any type of test sample. The article focuses on application of automated methods for FC sample preparation in 384-well assay plates. It also highlights a pipeline for analyzing large volumes of FC data, covering a visualization approach that facilitates review of screen-level data by dynamically embedding FlowJo (FJ) workspace images for each sample into a Spotfire file, directly linking them to the metric being observed. Finally, an application of these methods to a screen for MHC-I expression upregulators is discussed.

Item Type: Article
Keywords: flow cytometry high throughput phenotypic assay
Date Deposited: 26 Jun 2018 00:45
Last Modified: 25 Jan 2019 00:45
URI: https://oak.novartis.com/id/eprint/35213

Search

Email Alerts

Register with OAK to receive email alerts for saved searches.