Skilitsi, Anastasia Ioanna and Turko, Timothé and Cianfarani, Damien and Barre, Sophie and Uhring, Wilfried and Hassiepen, Ulrich and Léonard, Jérémie (2017) Towards Sensitive, High-Throughput, Biomolecular Assays Based on Fluorescence Lifetime. Methods and applications in fluorescence /. ISSN 2050-6120

Abstract

Time-resolved fluorescence detection for robust sensing of biomolecular interactions is developed by implementing Time Correlated Single Photon Counting in high-throughput conditions. Droplet microfluidics is used as a promising platform for the very fast handling of low-volume samples. We illustrate the potential of this very sensitive and cost-effective technology in the context of an enzymatic activity assay based on fluorescently-labeled biomolecules. Fluorescence lifetime detection by Time Correlated Single Photon Counting is shown to enable reliable discrimination between positive and negative control samples at a throughput as high as several hundred samples per second.

Item Type:	Article
Keywords:	Time-Resolved Fluorescence, Time Correlated Single Photon Counting, Biomolecular Interaction Assays, High-Throughput Screening, Droplet Microfluidics
Date Deposited:	27 Sep 2017 00:45
Last Modified:	27 Sep 2017 00:45
URI:	https://oak.novartis.com/id/eprint/33698