Browse views: by Year, by Function, by GLF, by Subfunction, by Conference, by Journal

Towards Sensitive, High-Throughput, Biomolecular Assays Based on Fluorescence Lifetime

Skilitsi, Anastasia Ioanna and Turko, Timothé and Cianfarani, Damien and Barre, Sophie and Uhring, Wilfried and Hassiepen, Ulrich and Léonard, Jérémie (2017) Towards Sensitive, High-Throughput, Biomolecular Assays Based on Fluorescence Lifetime. Methods and applications in fluorescence /. ISSN 2050-6120

Abstract

Time-resolved fluorescence detection for robust sensing of biomolecular interactions is developed by implementing Time Correlated Single Photon Counting in high-throughput conditions. Droplet microfluidics is used as a promising platform for the very fast handling of low-volume samples. We illustrate the potential of this very sensitive and cost-effective technology in the context of an enzymatic activity assay based on fluorescently-labeled biomolecules. Fluorescence lifetime detection by Time Correlated Single Photon Counting is shown to enable reliable discrimination between positive and negative control samples at a throughput as high as several hundred samples per second.

Item Type: Article
Keywords: Time-Resolved Fluorescence, Time Correlated Single Photon Counting, Biomolecular Interaction Assays, High-Throughput Screening, Droplet Microfluidics
Date Deposited: 27 Sep 2017 00:45
Last Modified: 27 Sep 2017 00:45
URI: https://oak.novartis.com/id/eprint/33698

Search

Email Alerts

Register with OAK to receive email alerts for saved searches.