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Data-analysis strategies for image-based cell profiling

Caicedo, Juan and Cooper, Sam and Heigwer, Florian and Warchal, Scott and Qiu, Peng and Molnar, Csaba and Vasilevich, Aliaksei and Barry, Joseph and Singh, Harmanjit and Kraus, Oren and Wawer, Mathias and Paavolainen, Lassi and Herrmann, Markus and Rohban, Mohammad and Hung, Jane and Hennig, Holger and Concannon, John and Smith, Ian and Singh, Shantanu and Rees, Paul and Horvath, Peter and Linington, Roger and Carpenter, Anne (2017) Data-analysis strategies for image-based cell profiling. Nature Methods, 14 (9). pp. 849-863. ISSN 15487105

Abstract

Image-based cell profiling is a high-throughput strategy for the quantification of phenotypic differences among a variety of cell populations. It paves the way to studying biological systems on a large scale by using chemical and genetic perturbations. The general workflow for this technology involves image acquisition with high-throughput microscopy systems and subsequent image processing and analysis. Here, we introduce the steps required to create high-quality image-based (i.e., morphological) profiles from a collection of microscopy images. We recommend techniques that have proven useful in each stage of the data analysis process, on the basis of the experience of 20 laboratories worldwide that are refining their image-based cell-profiling methodologies in pursuit of biological discovery. The recommended techniques cover alternatives that may suit various biological goals, experimental designs, and laboratories' preferences.

Item Type: Article
Date Deposited: 26 Sep 2017 00:45
Last Modified: 25 Jan 2019 00:45
URI: https://oak.novartis.com/id/eprint/32040

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