Stutz, Katharina , Hiss, Jan A., Schneider, Petra , Blatter, Markus, Pfeiffer, Bernhard , Posselt, Gernot and Schneider, Gisbert (2017) Peptide-membrane interaction between targeting and lysis. ACS Chemical Biology. ISSN 1554-89291554-8937

Abstract

Certain cationic peptides interact with biological membranes. These often-complex interactions can result in peptide targeting to the membrane, or in membrane permeation, rupture, and cell lysis. We investigated the relationship between the structural features of membrane-active peptides and these effects, to better understand these processes. To this end, we employed a computational method for morphing a membranolytic antimicrobial peptide into a mitochondrial targeting peptide by “directed simulated evolution”. The results obtained demonstrate that superficially subtle sequence modifications can strongly affect the peptides’ membranolytic and membrane-targeting abilities. Spectroscopic analyses suggest that N- and C-terminal structural flexibility plays a crucial role in determining the mode of peptide-membrane interaction.

Item Type:	Article
Date Deposited:	29 Aug 2017 00:45
Last Modified:	29 Aug 2017 00:45
URI:	https://oak.novartis.com/id/eprint/32031