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Application of Imaging Mass Spectrometry to Assess Ocular Drug Transit

Grove, Kerri and Kansara, Viralbhai and Prentiss, Melissa and Long, Debby and Mogi, Muneto and Kim, Sean and Rudewicz, Patrick (2017) Application of Imaging Mass Spectrometry to Assess Ocular Drug Transit. Journal of biomolecular screening. ISSN 1552-454X; 1087-0571

Abstract

MALDI imaging mass spectrometry (IMS) is becoming an important technology to determine the distribution of drugs and their metabolites in tissue of preclinical species after dosing. Interest in IMS is growing in the ophthalmology field, but little work to this point has been done to investigate ocular drug transit using this technology. Information on where and how a drug is distributing through the eye is important in understanding efficacy and whether it is reaching the desired target tissue. For this study, ocular distribution of brimonidine was investigated in rabbits following topical administration. Brimonidine has been shown to lower intraocular pressure and is approved to treat glaucoma, the second leading cause of blindness in the world. We have developed IMS methods to assess transit of topically administered brimonidine from the anterior to the posterior segment of rabbit eyes. Using IMS, brimonidine was detected in the cornea, aqueous humor, iris, and posterior segments of the eye. The distribution of brimonidine suggests that the route of transit following topical administration is mainly through the uvea-scleral route. This study demonstrates that IMS can be applied to monitor ocular transit and distribution of topically administered drugs.

Item Type: Article
Date Deposited: 18 Jan 2020 00:45
Last Modified: 18 Jan 2020 00:45
URI: https://oak.novartis.com/id/eprint/31035

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