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Dengue virus NS4B interacts with NS3 and dissociates it from single-stranded RNA.

Umareddy, Indira and Chao, Alex and Sampath, Aruna and Gu, Feng and Vasudevan, Subhash (2006) Dengue virus NS4B interacts with NS3 and dissociates it from single-stranded RNA. The Journal of General Virology, 87 (Pt 9). pp. 2605-2614. ISSN 0022-1317

Abstract

Dengue virus, a member of the family Flaviviridae of positive-strand RNA viruses, has seven non-structural proteins: NS1, NS2A, NS2B, NS3, NS4A, NS4B and NS5. Except for enzymic activities contained within NS3 and NS5, the roles of the other proteins in virus replication and pathogenesis are not well defined. In this study, a physical interaction between NS4B and the helicase domain of NS3 was identified by using a yeast two-hybrid assay. This interaction was further confirmed by biochemical pull-down and immunoprecipitation assays, both with purified proteins and with dengue virus-infected cell lysates. NS4B co-localized with NS3 in the perinuclear region of infected human cells. Furthermore, NS4B dissociated NS3 from single-stranded RNA and consequently enhanced the helicase activity of NS3 in an in vitro unwinding assay. These results suggest that NS4B modulates dengue virus replication via its interaction with NS3.

Item Type: Article
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Additional Information: free final full text version available at publisher's official URL; author can archive post-print (ie final draft post-refereeing); On author or institutional server only; Publisher's version/PDF cannot be used unless payment of fee
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Date Deposited: 14 Dec 2009 14:01
Last Modified: 31 Jan 2013 01:20
URI: https://oak.novartis.com/id/eprint/310

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