Dengue virus NS4B interacts with NS3 and dissociates it from single-stranded RNA.
Tools
Tools
Umareddy, Indira, Chao, Alex, Sampath, Aruna, Gu, Feng and Vasudevan, Subhash (2006) Dengue virus NS4B interacts with NS3 and dissociates it from single-stranded RNA. The Journal of General Virology, 87 (Pt 9). pp. 2605-2614. ISSN 0022-1317
Abstract
Dengue virus, a member of the family Flaviviridae of positive-strand RNA viruses, has seven non-structural proteins: NS1, NS2A, NS2B, NS3, NS4A, NS4B and NS5. Except for enzymic activities contained within NS3 and NS5, the roles of the other proteins in virus replication and pathogenesis are not well defined. In this study, a physical interaction between NS4B and the helicase domain of NS3 was identified by using a yeast two-hybrid assay. This interaction was further confirmed by biochemical pull-down and immunoprecipitation assays, both with purified proteins and with dengue virus-infected cell lysates. NS4B co-localized with NS3 in the perinuclear region of infected human cells. Furthermore, NS4B dissociated NS3 from single-stranded RNA and consequently enhanced the helicase activity of NS3 in an in vitro unwinding assay. These results suggest that NS4B modulates dengue virus replication via its interaction with NS3.
| Item Type: | Article |
|---|---|
| Related URLs: | |
| Additional Information: | free final full text version available at publisher's official URL; author can archive post-print (ie final draft post-refereeing); On author or institutional server only; Publisher's version/PDF cannot be used unless payment of fee |
| Related URLs: | |
| Date Deposited: | 14 Dec 2009 14:01 |
| Last Modified: | 31 Jan 2013 01:20 |
| URI: | https://oak.novartis.com/id/eprint/310 |