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Identification of a novel NAMPT inhibitor by CRISPR/Cas9 chemogenomic profiling in mammalian cells

Estoppey, David and Hewett, Jeffrey and Guy, Chantale and Harrington, Edmund and Thomas, Jason and Schirle, Markus and Cuttat-Theurillat, Rachel and Waldt, Annick and Gerrits, Bertran and Yang, Zinger and Schuierer, Sven and Carbone, Walter and Knehr, Judith and Lindeman, Alicia and Russ, Carsten and Frias, Elizabeth and Hoffman, Gregory and Varadarajan, Malini and Ramadan, Nadire and Reece-Hoyes, John and Wang, Qiong and Chen, Xin and Mcallister, Gregory and Roma, Guglielmo and Bouwmeester, Antonius and Hoepfner, Dominic (2017) Identification of a novel NAMPT inhibitor by CRISPR/Cas9 chemogenomic profiling in mammalian cells. Scientific reports, 7 (42728). pp. 1-6. ISSN 2045-2322

Abstract

Chemogenomic profiling is a powerful and unbiased approach to elucidate targets and mechanism of bioactive compounds. Until recently, high-quality experiments of this nature have been limited to fungal systems due to lack of mammalian genome-wide deletion collections. Here we show that the CRISPR/Cas9 system enables the generation of such libraries and allows for the identification of targets and pathways mediating hypersensitivity and resistance relevant to the compound mechanism of action, using a novel NAMPT inhibitor as an example.

Item Type: Article
Date Deposited: 22 Feb 2017 00:45
Last Modified: 22 Feb 2017 00:45
URI: https://oak.novartis.com/id/eprint/30845

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