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GNF ID 100504: The crystal structure of Neisseria gonorrhoea NGO1391 defines a novel set of bacterial proteins that are similar to the N-terminal domain of human Sufu (Suppresor of Fused)

Abdubek, Polat and Chen, Connie and Chiu, Michelle and Feuerhelm, Julie and Grant, Joanna and Klock, Heath and Knuth, Mark and Nigoghossian, Edward and Okach, Linda and Puckett, Christina and Lesley, Scott and Stolz, Barbara (2010) GNF ID 100504: The crystal structure of Neisseria gonorrhoea NGO1391 defines a novel set of bacterial proteins that are similar to the N-terminal domain of human Sufu (Suppresor of Fused). Protein Science or Journal of Bacteriology.

Abstract

Sufu (Suppresor of Fused), a two domain protein, plays a critical role in regulating Hedgehog signaling and is conserved from flies to humans. A few bacterial Sufu-like proteins have previously been identified based on sequence similarity to the N-terminal domain of eukaryotic Sufu proteins, but none of them have been structurally or biochemically characterized and their function in bacteria is unknown. We have determined the crystal structure of a more distantly related Sufu-like homolog, NGO1391 from Neisseria gonorrhea, at 1.4 Å resolution, which provides the first biophysical characterization of a bacterial Sufu-like protein. The structure revealed a striking similarity to the N-terminal domain of human Sufu, despite an extremely weak sequence identity of ~15%. Subsequent sequence analysis revealed that NGO1391 defines a new set of smaller Sufu-like proteins from ~200 bacterial species and has resulted in an expansion of the Sufu family in Pfam.

Item Type: Article
Additional Information: GNF publication ID 100504. This publcation is a grant requirement. B. Stolz is NOT an author -- NVS contact person for GNF manuscript approval. This manuscript shall NOT be copied to the OAK internet.
Date Deposited: 13 Oct 2015 13:16
Last Modified: 13 Oct 2015 13:16
URI: https://oak.novartis.com/id/eprint/3064

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