Abdubek, Polat, Chen, Connie, Chiu, Michelle, Feuerhelm, Julie, Grant, Joanna, Klock, Heath, Knuth, Mark, Nigoghossian, Edward, Okach, Linda, Puckett, Christina, Lesley, Scott and Stolz, Barbara (2010) GNF ID 100504: The crystal structure of Neisseria gonorrhoea NGO1391 defines a novel set of bacterial proteins that are similar to the N-terminal domain of human Sufu (Suppresor of Fused). Protein Science or Journal of Bacteriology.

Abstract

Sufu (Suppresor of Fused), a two domain protein, plays a critical role in regulating Hedgehog signaling and is conserved from flies to humans. A few bacterial Sufu-like proteins have previously been identified based on sequence similarity to the N-terminal domain of eukaryotic Sufu proteins, but none of them have been structurally or biochemically characterized and their function in bacteria is unknown. We have determined the crystal structure of a more distantly related Sufu-like homolog, NGO1391 from Neisseria gonorrhea, at 1.4 Å resolution, which provides the first biophysical characterization of a bacterial Sufu-like protein. The structure revealed a striking similarity to the N-terminal domain of human Sufu, despite an extremely weak sequence identity of ~15%. Subsequent sequence analysis revealed that NGO1391 defines a new set of smaller Sufu-like proteins from ~200 bacterial species and has resulted in an expansion of the Sufu family in Pfam.

Item Type:	Article
Additional Information:	GNF publication ID 100504. This publcation is a grant requirement. B. Stolz is NOT an author -- NVS contact person for GNF manuscript approval. This manuscript shall NOT be copied to the OAK internet.
Date Deposited:	13 Oct 2015 13:16
Last Modified:	13 Oct 2015 13:16
URI:	https://oak.novartis.com/id/eprint/3064