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Filterable Agents for Hyperpolarization of Water, Metabolites, and Proteins

Vuichoud, Basile and Bornet, Aurélien and de Nanteuil, Florian and Milani, Jonas and Canet, Estel and Ji, Xiao and Mieville, Pascal and Weber, Emmanuelle and Kurzbach, Dennis and Flamm, Andrea and Konrat, Robert and Gossert, Alvar and Jannin, Sami and Bodenhausen, Geoffrey (2016) Filterable Agents for Hyperpolarization of Water, Metabolites, and Proteins. Chemistry - a European journal, 22 (41). pp. 14696-14700. ISSN 09476539

Abstract

Hyperpolarization is generated by dissolution dynamic nuclear polarization using a polymer-based polarizing agent dubbed FLAP (Filterable Labeled Agents for Polarization). It consists in a thermo-responsive poly(N-isopropylacrylamide), also known as pNiPAM-COOH, labelled with nitroxide radicals. The polymer powder is impregnated with an arbitrary solution of interest and frozen as is. Dissolution dynamic nuclear polarization (d-DNP) is followed by a simple filtration leading to hyperpolarized solutions free from any contaminants. We demonstrated the use of FLAP to hyperpolarize partially deuterated water up to P(1H) = 6 % with a long relaxation T1 > 36 s characteristic of high purity. Water hyperpolarization can be transferred to drugs, metabolites or proteins that are waiting in an NMR spectrometer, either by exchange of labile protons or through intermolecular Overhauser effects. We also show that FLAP are also suitable polarizing agents for 13C-labeled metabolites such as pyruvate, acetate and alanine.

Item Type: Article
Date Deposited: 12 Oct 2016 00:45
Last Modified: 12 Oct 2016 00:45
URI: https://oak.novartis.com/id/eprint/30200

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