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Fam60A plays a role for production stabilities of recombinant CHO cell lines

Ritter, Anett and Nuciforo, Sandro and Schulze, Axel and Oertli, Mevion and Rauschert, Tatjana and Voedisch, Bernd and Geisse, Sabine and Jostock, Thomas and Laux, Holger (2016) Fam60A plays a role for production stabilities of recombinant CHO cell lines. Biotechnology and bioengineering, n.a. (n.a.). n.a.-n.a.. ISSN 00063592

Abstract

Recombinant CHO (Chinese hamster ovary) cell lines producing therapeutic proteins often lose their production capability during long-term cultivation. To ensure that CHO production cell lines can be up-scaled to high volume bioreactors labor intensive stability studies of several months have to be performed to deselect clones that are losing productivity over time. The ability to predict whether clones will produce recombinant proteins at constant high levels e.g. through determination of biomarkers such as expression of specific genes, plasmid integration sites or epigenetic patterns, or even to improve CHO host cell lines to increase the probability of the generation of stable clones would be highly beneficial. Previously, we reported that the lack of a telomeric region of chromosome 8 correlates with increased productivities and higher production stabilities of monoclonal antibody expressing CHO cell lines (Ritter et al. 2015). Herein, we describe that the knock-out of the gene Fam60A, which is one of the genes located within the telomeric region of chromosome 8, in CHO-K1a cells leads to the isolation of significantly more clones with higher protein production stabilities of monoclonal antibodies during long-term cultivation.

Item Type: Article
Date Deposited: 25 Sep 2016 00:45
Last Modified: 25 Sep 2016 00:45
URI: https://oak.novartis.com/id/eprint/29236

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