Browse views: by Year, by Function, by GLF, by Subfunction, by Conference, by Journal

Recombinase-based reporter system and antisense technology to study gene expression and essentiality in hypoxic nonreplicating mycobacteria.

Rao, Srinivasa and Camacho, Luis and Tan, Bee Huat and Boon, Calvin and Russel, David G and Dick, Thomas and Pethe, Kevin (2008) Recombinase-based reporter system and antisense technology to study gene expression and essentiality in hypoxic nonreplicating mycobacteria. FEMS Microbiology Letters, 284 (1). pp. 68-75. ISSN 0378-1097

Abstract

The study of the mechanisms used by Mycobacterium tuberculosis to survive in the absence of growth is hampered by the absence of appropriate genetic tools. Here, we report two strategies, a recombinase-based reporter system and an antisense technology, to study gene expression and essentiality in hypoxic nonreplicating mycobacteria. The recombinase-based reporter system relies on the resolution of an antibiotic marker flanked by the gammadelta-res sites. This system was developed to identify M. tuberculosis promoters, which are specifically expressed under anaerobic conditions. The antisense strategy was designed to study the role of a gene candidate during anaerobic survival. To validate this approach, the dosR, narK2 and rv2466c promoters were selected to drive dosR antisense mRNA expression in quiescent mycobacteria. The conditional knockout strains were found to be attenuated to adapt and survive under anaerobic conditions, as observed for the dosR knockout strain. Together, our work demonstrates that the recombinase-based reporter system and antisense technology represent two genetic tools useful for the identification and characterization of genes essential for the survival of hypoxic nonreplicating M. tuberculosis.

Item Type: Article
Related URLs:
Additional Information: archiving not allowed on institutional repository
Keywords: Mycobacterium tuberculosis; hypoxic dormancy; antisense; resolvase
Related URLs:
Date Deposited: 14 Dec 2009 14:02
Last Modified: 31 Jan 2013 01:21
URI: https://oak.novartis.com/id/eprint/291

Search

Email Alerts

Register with OAK to receive email alerts for saved searches.