Gualtieri, Giovanna (2014) Effects of cigarette smoke on Toll-like receptor (TLR) activation of chronic obstructive pulmonary disease (COPD) macrophages. Clinical and Experimental Immunology, 176 (3). pp. 461-472. ISSN 0009-9104; 1365-2249

Abstract

Chronic obstructive pulmonary disease (COPD) is characterized by an abnormal innate immune response. We have investigated the changes in the innate immune response of COPD alveolar macrophages exposed to both cigarette smoke and Toll-like receptor (TLR) stimulation. COPD and control alveolar macrophages were exposed to cigarette smoke extract (CSE) followed by TLR-2, -4 and -5 ligands [Pam3CSK4, lipopolysaccharide (LPS) and phase I flagellin (FliC), respectively] or non-typeable Haemophilus influenzae (NTHi). CSE exposure suppressed TLR-induced tumour necrosis factor (TNF)-alpha, interleukin (IL)-6, IL-10 and regulated on activation, normal T cell expressed and secreted (RANTES) production in both COPD and control alveolar macrophages, but had no effect on interleukin 8 (CXCL8) production. Similarly, CSE suppressed NTHi-induced TNF-alpha but not NTHi-induced CXCL8 production in COPD alveolar macrophages. Gene expression analysis showed that CSE suppressed LPS-induced TNF-alpha transcription but not CXCL8 transcription in COPD alveolar macrophages. The dampening effect of CSE on LPS-induced cytokine production was associated with a reduction in p38, extracellular signal regulated kinase (ERK) and p65 activation. In conclusion, CSE caused a reduced innate immune response in COPD alveolar macrophages, with the exception of persistent CXCL8 production. This could be a mechanism by which alveolar macrophages promote neutrophil chemotaxis under conditions of oxidative stress and bacterial exposure. © 2014 British Society for Immunology.

Item Type:	Article
Additional Information:	Unmapped bibliographic data: LA - English [Field not mapped to EPrints] ST - Effects of cigarette smoke on Toll-like receptor (TLR) activation of chronic obstructive pulmonary disease (COPD) macrophages [Field not mapped to EPrints] AU - Metcalfe, H. J. [Field not mapped to EPrints] AU - Lea, S. [Field not mapped to EPrints] AU - Hughes, D. [Field not mapped to EPrints] AU - Khalaf, R. [Field not mapped to EPrints] AU - Abbott-Banner, K. [Field not mapped to EPrints] AU - Singh, D. [Field not mapped to EPrints] AD - (Metcalfe, Lea, Hughes, Khalaf, Singh) University of Manchester, Manchester Academic Health Science Centre, University Hospital South Manchester NHS Foundation Trust, NIHR South Manchester Respiratory and Allergy Clinical Research Facility, Manchester, United Kingdom [Field not mapped to EPrints] DB - Embase [Field not mapped to EPrints] DP - Ovid Technologies [Field not mapped to EPrints] AN - 2014284858 [Field not mapped to EPrints]
Keywords:	Bacteria
Date Deposited:	11 Nov 2016 00:45
Last Modified:	11 Nov 2016 00:45
URI:	https://oak.novartis.com/id/eprint/28968