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A Fluorescence Lifetime-Based Assay for Abelson Kinase

Pritz, Stephan, Meder, Gabriele, Doering, Klaus, Drueckes, Peter, Woelcke, Julian, Mayr, Lorenz and Hassiepen, Ulrich (2011) A Fluorescence Lifetime-Based Assay for Abelson Kinase. JOURNAL OF BIOMOLECULAR SCREENING, 16 (1). pp. 65-72. ISSN 1087-0571


We present a novel homogenous in vitro assay format, and apply it to the quantitative determination of the enzymatic activity of a tyrosine kinase. A single probe attached to a peptidic substrate responds with changes in its fluorescence lifetime, depending on whether or not a nearby tyrosine is phosphorylated. We utilize this effect to directly follow the enzymatic phosphorylation of the substrate, without having to resort to additional assay components such as antibodies. As an example for the application of this assay principle, we present results from the development of an assay for Abelson kinase (c-Abl) used for compound profiling. Adjustments in the peptide sequence would make this assay suitable to a wide variety of other tyrosine kinases.

Item Type: Article
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Additional Information: author can archive post-print (ie final draft post-refereeing); Publisher's version/PDF cannot be used
Keywords: kinase, c-Abl, assay, fluorescence liftime, screening, enzyme activity
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Date Deposited: 13 Oct 2015 13:16
Last Modified: 13 Oct 2015 13:16


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